TY - JOUR AU - Sanjit Mukherjee AU - Sweta Mohanty AU - Atul Katarkar AU - Richa Dhariwal AU - Basudev Mahato AU - Jay Gopal Ray AU - Keya Chaudhuri PY - 2021/07/09 Y2 - 2024/03/28 TI - Association of NQO1 C609T (Pro187Ser) with Risk of Oral Submucous Fibrosis in Eastern Indian Population JF - Asian Pacific Journal of Cancer Biology JA - apjcb VL - 6 IS - 3 SE - Research Articles/ Original Work DO - 10.31557/apjcb.2021.6.3.187-193 UR - http://waocp.com/journal/index.php/apjcb/article/view/683 AB - Objective: Oral submucous fibrosis (OSF) is a debilitating disease mainly attributed to chewing areca nut with a 7.4-13% malignant transformation rate. The present study explores the role of NADPH quinone oxidoreductase 1 (NQO1) C609T (Pro187Ser) polymorphism in susceptibility to OSF among habitual areca nut chewers in an eastern Indian population. Material and Methods: In this hospital-based study, 152 controls and 179 OSF cases were genotyped at NQO1 C609T polymorphic site by PCR-RFLP and its effect on NQO1 expression in OSF tissue was studied to determine the risk of the disease. Results: Overall, about 18% of the total OSF cases were detected carrying minor TT alleles (Ser/Ser) p=0.026. When categorized by age, both CT (Pro/Ser) and TT (Ser/Ser) alleles were significantly higher (p= 0.003 & 0.004 respectively) in cases above 40years of age. NQO1 protein was 42% reduced in buccal tissues of heterozygous (Pro/Ser) carriers, whereas a 70% reduction was observed in TT (Ser/Ser) OSF cases. Conclusion: Our study suggests that the NQO1 C609T polymorphism confers increased risk for OSF in habitual chewers.  ER -