Asian Pacific Journal of Cancer Biology 2019-10-13T18:22:07+01:00 Hoda Golmahi Open Journal Systems <p><em>The Asian Pacific Journal of Cancer Biology (APJCB) is an open access electronic journal,&nbsp;which covers all aspects related to cancer biology. </em><em>&nbsp;</em><em>The journal was launched in 2016 as the official publication of the West Asia Organization for Cancer Prevention (WAOCP) .&nbsp; All manuscripts published in the Asia Pacific Journal of Cancer Biology, are under the terms of the Creative Commons Attribution License. This permits anyone to copy, distribute, transmit and adapt the published work, provided the original work and source are appropriately cited.</em></p> Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma: A Focus on Tumor-Infiltrating Lymphocytes 2019-10-13T18:22:07+01:00 Marzieh Norouzian Sima Balouchi-Anaraki <p>For more progress in head and neck squamous cell carcinoma (HNSCC) immuno-oncology, further understanding of interactions between tumor and immune system as well as factors in the tumor microenvironment is required. HNSCC is seriously infiltrated by lymphocytes but is known to be highly immunosuppressive. The aim of this review is to highlight the complexity of tumor microenvironment and tumor- immune cells interaction in the HNSCC, in order to improve understanding of tumorigenesis and disease progression in HNSCC patient and to provide valuable information about prognostic markers. The main goal of this review is to discuss the role of the tumor infiltrating lymphocytes in tumor progression, their cross-talk with other components of the tumor microenvironment as well as their roles in carcinogenesis, metastasis process, treatment, and prognosis in head and neck squamous cell carcinomas.</p> 2019-08-01T00:00:00+01:00 ##submission.copyrightStatement## Chrysin Sensitizes Human Lung Cancer Cells to Tumour Necrosis Factor Related Apoptosis-Inducing Ligand (TRAIL) Mediated Apoptosis 2019-10-13T18:22:06+01:00 Syed Hassan Mehdi Md Zafaryab Sana Nafees Asad Khan Irfan Ahmad Zubair Bin Hafeez Moshahid Alam Rizvi <p><strong>Background:</strong> Lung cancer is the primary cause of cancer deaths worldwide. Thus, the requisite for more coherent methods to lung cancer therapy is needed. <br><strong>Purpose:</strong> Chrysin (5, 7-dihydroxyflavone) is a naturally occurring flavonoid having a wide range of pharmacological properties and is commonly found in fruits, vegetables, honey and propolis. In our study, we have hypothesized that chrysin would have anticancer activity on L132 lung cancer cell line.<br><strong>Methods:</strong> The cytotoxic effects were assessed by MTT and NRU assay. DAPI was used to evaluate the cell death. The pro- or anti-apoptotic proteins were detected by Western Blot assay, and, besides, mRNA expression was analysed with RT-PCR. In silico study of chrysin was performed to identify suitable inhibitors against the protein function. <br><strong>Results:</strong> Results indicated that chrysin enhanced the inhibitory effects of TRAIL (Tumour Necrosis Factor Related Apoptosis-Inducing Ligand) in comparison to TNF-α (tumour necrosis factor) on cell viability in L132 lung cancer cells and altered nuclear morphology of cells was observed in DAPI (4’,6-diamidino-2-phenylindole) staining after 48 hrs treatment. Treatment with chrysin enhances TRAIL-induced apoptosis by increasing the expression of apoptosis-related proteins including caspase-3, 8, 9 and Bax, whereas the expression of Bcl-2 was decreased. Chrysin was docked with caspase-3, 8, 9, Bax, and Bcl-2 proteins to identify suitable inhibitors against the protein function.<br><strong>Conclusion:</strong> We concluded that chrysin sensitizes lung cancer cells to TRAIL-induced apoptosis and may be considered for future studies as a promising therapeutic candidate for human lung cancer.</p> 2019-08-03T11:35:56+01:00 ##submission.copyrightStatement##