CRISPR-Cas9: An Evolutionary Technique for the Treatment of Breast and Cervical Carcinoma

Abstract

CRISPR/Cas9 has transformed genome editing methods in several scientific domains, including the study of human cancer. Globally, breast cancer (BC) affects women more than any other kind of cancer and triple-negative breast cancer (TNBC) has the greatest fatality rate. The diagnosis and treatment of breast cancer have come a long way, yet many patients still experience metastases or relapses. One of the potential techniques for correcting faulty genes and curing different cancers is gene therapy. Certain mutations associated with cancer onset and development might be repaired in model organisms as a first step toward translational applications using the CRISPR/Cas9 technology. The CRISPR/Cas9 system, which consists of a single readily modified guide RNA (sgRNA) sequence coupled to a Cas9 nuclease, has transformed genome editing owing to its simplicity and effectiveness when compared to previous methods. CRISPR/Cas9 allows for the knockdown of over-expressed genes, the reversal of mutations in defected genes, and the remodeling of the regulatory environment to inhibit BC proliferation. CRISPR/Cas9-based treatment mixed with traditional chemotherapy has proven to be beneficial in combating drug resistance and tumor inhibition difficulties. The CRISPR cas9 system may edit many genes at the same time by generating numerous sgRNAs that target different genomic regions, considerably increasing its therapeutic potential and making it a unique technique for treating cervical lesions. This review focuses on the therapeutic applications of CRISPR/Cas9 in the treatment of cervical cancer and breast cancer.

Introduction

Numerous novel gene editing techniques have been discovered in the last 10 to 20 years and are currently being employed in a variety of applications. The CRISPR/ Cas9 system stood out among all of those techniques as a pioneering technique that allows the insertion, deletion, and correction of genetic material both in vivo and in vitro. The medical and health fields have been significantly transformed by this method [1]. Using a guided RNA to locate its target sequence, this method subsequently inserts or deletes a DNA segment with the aid of the Cas9 enzyme [2]. The natural repair systems of the cells, homology-directed repair (HDR) and non-homologous end joining (NHEJ), are triggered when the cleavage occurs [3, 4]. It is possible to insert donor DNA to serve as a template at the location of cleavage by using the right mechanism and design.

The second most frequent cancer in women to cause mortality worldwide is breast cancer [5, 6]. Breast cancer accounts for 28% of all new cancer cases [7]. It develops silently and is detected on routine screening. Both the initiation and development of breast cancer involve several signaling pathways [8]. Studies have revealed that the expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) is linked to a number of breast cancer subtypes [9]. Some molecular markers associated with breast cancer are represented in Figure 1.

Figure 1. Molecular Subtypes of Breast Cancer.

Adverse physical side effects, non-specificity and drug resistance are major problems even if there aren’t many medications licensed for the treatment of breast cancer [10, 11]. CRISPR/Cas9 technology is getting a lot of interest as research on breast cancer continues to be a hot topic.

CRISPR/Cas9 is the newest type of gene editing technology among all available techniques, yet research has shown that it is also the most effective one. This technique does have some drawbacks as well; however, researchers are striving to address these limitations [12]. The CRISPR-CAS system is valuable for studies on cancer, particularly breast cancer [13]. The therapeutic promise of the CRISPR/Cas9 system against breast and ovarian cancer is highlighted in this review. We will explain how the CRISPR/Cas9 system can be used to overcome drug resistance in breast and ovarian cancer. We’ll discuss about the CRISPR/Cas9 system’s limitations, advances, and the future prospects in the final section.

CRISPR-CAS: A gift from the nature

The eukaryotic genome contains millions of bases that are hard to modify, but efforts are being made to find new anticancer targets and design new therapeutic approaches. The natural DNA repair machinery is stimulated by double stranded bricks (DSBs) in the DNA, according to studies. By using non-homologous end joining (NHEJ), mutant sequence can be added to or removed from the cleavage site. However, homology directed repair (HDR) in animal models permits both knock-in and knock-out (Figure 2).

Figure 2. Genome Editing Using Natural Repair Mechanism of Host.

These investigations aided in the discovery of numerous novel gene editing tools [11].

Prokaryotes’ defense mechanism led to the discovery of the CRISPR/Cas9 system, which turned out to be a gift from nature. CRSIPR-CAS was first identified in the 1980s as a prokaryote defense system, but it wasn’t until 2012 that its potential for use as a gene editing system was investigated. Bacteria’s CRISPR-CAS system cleaves foreign genetic material and functions as an immune system. Using guided RNA and the CAS9 protein, the CRISPR/Cas9 system can induce double stranded bricks on the targeted sites of the genome [14, 15]. The CRISPR- Cas9 system needs a protospacer adjacent motif (PAM) next to the targeted region in order to recognize and cleave the DNA [16, 17]. Regarding the CRISPR-Cas9 mechanism, 60% of archaea and 40% of bacteria show minor differences [18]. The spacers in the CRISPR locus act as memory repositories and shield the host from attacks from the same invader over and over again.

CRISPR/Cas9 system is widely used in the breast cancer research due to its effectiveness. The effectiveness of the CRISPR/Cas9 system is due to its ease of use. In comparison to previous methods, the CRISPR/Cas9 system is simpler to construct and less expensive. The PAM requirement is a restriction, although this can be bypassed by employing different CAS proteins. The bases and methylation patterns can be altered with the help of enzymes like DNA methyltransferases (DNMT) and DNA deaminases. Such modifications can improve the therapeutic potential of the CRISPR/Cas9 system in the treatment of diseases like breast cancer. Some Targeted genes of different cell types in animals and vector used are being represented in Table 1.

Table 1. Some Targeted Genes of Different Cell Types in Animals and Vector Used are Being Represented in This Table.

Sr.	Cell Type	Animal	Vector	Genes Targeted
1	Germline	Mouse	mRNA,sgRNA	Tet1, Tet2
		Rat	mRNA,sgRNA	Not Specific
		Monkey	mRNA,sgRNA	Pparg, Rag1
		Mouse	Plasmid DNA	p53, pten, APC
2	Somatic	Mouse	AAV	Mecp2
		Mouse	sgRNA	Icam2
		Mouse	Lentivirus	APC, pten
		Mouse	Adenovirus	Eml4-Alk
		Mouse	Plasmid DNA	Pten, p53, β-catenin
		Mouse	AAV	NeUN
		Mouse	Adenovirus	Cebpa
3	Lymphoma	Mouse	Lentivirus	Tet2, NF1, EZH2,RUNX1
		Mouse	Lentivirus	Mcl, p53
		Mouse	Retrovirus	p53
		Mouse	DNA electroporaion	Mll3

Tumor Modeling Via CRISPR-Cas System:

Converting a normal cell into a tumor cell is a multistep procedure in which the cell must go through a number of mutations [19, 20]. Scientists were successful in modeling tumor cells by inducing mutation at great speed and precision using the CRISPR/Cas9 system. Some studies reported that they have successfully applied this technique to model liver cancer cells by inducing mutations in cancer associated genes in vivo.

Successfully truncating the APC tumor suppressor gene in gut cells was indicative of a well-developed early event in the genesis of colorectal cancer. In order to choose APC-deficient human intestinal stem cells, the Wnt signaling activators have been removed from the growth media. This has induced β-catenin stability and up-regulation of the Wnt pathway [21, 22]. Other methods involve producing cells that have both a loss-of-function mutation and the KRAS oncogene active. Additionally, CRISPR/Cas9 was used to deactivate P53 [23]. These methods might also be used to create a mixture of mutations, which could then be investigated by injecting these modified cells into immunodeficient mice. The big question of whether the cancer-causing mutations happen randomly or in a specific order of time is answered by this cancer model.

Similar techniques have been utilized to use CRISPR/ Cas9, which targets multiple protein-coding genes as well as miRNA precursors, to transform a non-metastatic mouse lung tumor cell line. After injecting the transformed cells into immunodeficient mice, it was observed a large increase in tumor and lung metastasis [24]. The scientists used deep sequencing to identify numerous new genes whose expression was essential for tumor development and metastasis. This method enables the recapitulation of the development and metastasis of tumors, which may aid in the development of particular treatments that specifically target defective genes [25].

CRISPR/Cas9-mediated targeting human papillomavirus E6

The RNA guided endonuclease CRISPR-Cas9 is developed from the bacteria Streptococcus pyogenes. It has been widely employed for genome engineering in a range of species, as well as the genus plasmodium Leishmania, the underlying cause of human leishmaniasis, because of its simplicity, adaptability, and high effectiveness. CRISPR- Cas9 has been demonstrated to be a more efficient tool for deleting or disrupting Leishmania genes, generating point mutations, and adding tags to endogenous genes than classical homologous recombination gene targeting. The consistent CRISPR expression systems were demonstrated to eliminate multigene family Leishmania genes as well as genes contained in multiploid chromosomes, identify the critical Leishmania genes, and construct particular chromosomal translocations [26]. Cervical cancer caused by the human papillomavirus (HPV) is a serious health concern for women in developing countries. As its diagnosis and poor prognosis, it has a significant fatality rate. The development and major factor that leads to this kind of cancer are entirely dependent on two main oncogenes, E6 and E7, which are constitutively expressed, leading to carcinogenesis [27].

Human papillomaviruses of the Papillomaviridae family are a kind of tiny non-enveloped circular double-stranded DNA virus that measures 50-55 nm in diameter. The category has 300 distinct genotypes, 200 of which are believed to be harmful to humans. The scientists categorized papillomaviruses into genera, species, kinds, and subtypes based on gene sequences similarity of the L1 ORFs of 118 papillomavirus types (Pal & Kundu, 2020). Human papillomaviruses have been classified into five genera:

1. alpha (65 types including HPV16, 18, 31, 33, etc.)

2. beta (53 types including HPV5, 9, 49, etc.

3. gamma (98 types including HPV4, 48, 50, etc.)

4. mu (3 types including HPV1, HPV63, and HPV 204)

5. nu (HPV41). Among them, alpha-papillomaviruses are the most commonly focused group of papillomaviruses since they are known to be responsible for 5% of cancer occurrences worldwide [27].

The fourth most frequent cancer among women globally is cervical cancer [28]. The most prevalent high- risk HPV type and the one that carries the highest risk of developing cervical cancer is human papillomavirus type 16 (HPV16). In their lifetime, over than 80 percent of women who had at least one partner of the other sex will get HPV [29]. Persistent high-risk HPV infection has been identified as a major factor in the development of cervical cancer. Additionally, it was discovered by researchers that the HPV viruses can incorporate their DNA into the human genome, which appeared to be a crucial step in the development of carcinogenesis [30]. The HPV oncogene is persistently expressed as a result of integration, making it challenging to eradicate. For individuals with a chronic HPV infection and the incorporation of HPV genes, there is currently no viable therapy. Zinc finger nuclease (ZFN), transcription activator-like effectors nuclease (TALEN), and clustered regularly interspaced short palindrome repeat (CRISPR/Cas9) are the three primary gene-editing techniques. All of these techniques for gene editing may cause DNA double-strand breaks (DSBs) that were specifically targeted and modify genes by triggering DNA repair processes. Gene therapy is getting more accurate and efficient with the advancement of these gene-editing techniques [31]. These gene-editing strategies made targeting HPV oncoprotein genes might have a significant impact on the targeted cells. The effectiveness of gene treatments in treating HPV infection sickness has not yet been compared. Researchers just need to create the gRNA complementary to such target DNA sequence for the CRISPR/Cas9 system; no further components are required. Because it is quick and simple to create, the CRISPR/Cas9 system may be an appropriate substitute for ZFN and TALEN for initiating targeted gene editing. It might result in gene deletion, reversion, and insertion by causing DNA damage at a specific spot, which could then be repaired by the cell’s self-repairing machinery by NHEJ (non-homologous end-joining) or HDR (homologous-dependent repair). According to some earlier research, the HPV oncogene targeted by the CRISPR/Cas9 system may be able to treat HPV-induced cervical cancer. However, there hasn’t been much dynamic monitoring of the therapy process up to this point in the suitable animal model.

Protein degradation in breast cancer

A type of cancer that originates in breast cells is called breast cancer. Some of the signs and symptoms of breast cancer include:

• A newly reversed nipple

• A breast lump or thickening that feels different from the surrounding tissue

• A change in a breast’s size, shape, or appearance

• Variations in the skin covering the breast, such as dimpling;

• Peeling, scaling, crusting, or flaking of the pigmented place of skin around the nipple (areola) or breast skin;

• A newly inverted nipple; [32]

Both the rates of synthesis and the rates of degradation affect the number of proteins within cells. Asymmetric rates of protein breakdown are a key component of cell control. The ½ of proteins inside cells can range greatly, from a few minutes to many days. Many proteins that degrade quickly serve as regulatory molecules, including transcription factors. These proteins must have a quick turnover in order for their levels to react swiftly to outside stimuli. Another way for controlling the activity of intracellular enzymes is provided by the fast degradation of other proteins in response to certain signals. Furthermore, defective or damaged proteins are identified and quickly destroyed within cells, eradicating the effects of errors produced during protein synthesis. Protein breakdown in eukaryotic cells is mediated by two main pathways: lysosomal proteolysis and the ubiquitin-proteasome system.

The enhanced action of protein degradation is one of the key factors in promoting tumor cell growth. The 26S proteasome, an enzyme that is important for protein degradation including cell cycle control and apoptosis-related proteins, is one of these protein-degrading enzymes [33, 34]. Proteasome inhibitors have been shown to have anticancer and apoptosis-enhancing effects in cancer model organisms. Additionally, it makes tumor cells more susceptible to both intrinsic and extrinsic pro-apoptotic signals. As a result, the proteasome is now a target for cancer therapies. There is evidence that a site-specific protease phosphorylation mechanism regulates the growth of breast cancer [35], suggesting that disrupting and interfering with this mechanism might be useful in containing the illness. To stop the carcinogenesis of mice with proteasome-dependent human breast cancer cells, dual-specificity tyrosine-regulated kinase 2 (DYRK2) deletion (the proteins that phosphorylate the proteasome components) was developed [36]. Treatment options for ER-positive breast cancer include utilizing aromatase inhibitors (AI) to prevent the production of estrogen, as well as tamoxifen and fulvestrant, which compete with estrogen for ER. AI and tamoxifen are ineffective against advanced metastatic breast carcinoma caused by ER mutations like ERY537S and ERD538G [37]. ERY537S or ERD538G was substituted for the wild-type form of ER in a breast cancer positive ER model using CRISPR/Cas9 to demonstrate the impact of these mutations. These mutant cells exhibit estrogen independent behavior and are somewhat resistant to antiestrogen. It was discovered that the mutant cells’ antiestrogen resistance was connected to an increase in the polypeptide response that lessens ER degradation [38]. Breast cancer metastasis and cancer progression are influenced by migration and invasion enhancers (MIEN1). According to research, increasing MIEN1 expression may promote tumor spread and migration. Through the use of CRISPR/Cas9, a specific removal in this gene successfully reversed its expression, therefore controlling the disease’s progression. This method enables us to comprehend the part MIEN1 plays in the development of cancer and tumors in great detail, which may one day lead to the development of a breast cancer treatment option [39]. PTEN (phosphatase and tensin homolog) gene mutations are a critical stage in the development of cancer. PTEN is a tumor suppressor gene that regulates the cell cycle and regulates the rate of proliferation. In order to disrupt PTEN in mice with mammary gland-specific lack of E-cadherin, invasive lobular breast cancer (ILC)-initiating cells were specifically targeted using CRISPR/Cas9. This method may be used to quickly test in vivo potential tumor suppressor genes. ILC [40].

Use of CRISPR cas9 system for countermanding drugs resistance in cervical cancer:

Chemotherapy is currently the most viable cervical cancer treatment. A significant obstacle in cervical cancer treatment is cellular resistance, which develops as a result of the prolonged use of chemotherapeutic medications. The human papillomavirus (HPV) transmission, which results in the integration of the virus’ oncogenes E6 and E7 into host cells, is the primary cause of the significant number of cervical malignancies [41]. Therefore, the element for inducing and retaining cervical cancerization is the over expression of these two genes [42]. They provide the novelty and security of the nanoscale editing method as the HPV-derived E6 and E7 and are present in the cells of cervical cancer only [43].

The chemotherapeutic drug Docetaxel (DOC) and the CRISPR/Cas9 system were employed in conjunction to overcome the resistance to transformational therapy and enhance the therapeutic effect. In the cervical cancer model of the mouse, the nano-system created by encapsulating hydrophobic DOC negatively charged CRISPR/Cas9 plasmid, and cationic liposomes demonstrated great therapeutic efficacy and few adverse effects [44]. In order to prevent cervical cancer from growing and spreading from the start, the E6 and E7 oncogenes deletion is intended. The attacking of the E7 gene of HPV increased the expression level of Rb protein, which was found to increase 2.58-fold and 2.42-fold in expression. HPV E6 protein processes suppress the p53 tumor suppressor expression of the host, so the decline of E6 activity is anticipated to end in the stimulation of the expression of p53. Chemo resistance is significantly influenced by pRB and p53. After eliminating the HeLa cell’s E6 and E7 genes, the CRISPR system increased the pRB and p53 gene expression, which turns to induce HeLa cells to undergo apoptosis [45]. Some CRISPR approaches along with their target genes are being represented in Table 2.

Table 2. Some CRISPR Approaches along with Their Target Genes are Being Represented.

Target Genes	Cell line	CRISPR approach	References
FASN	MCF-7	Type 2 CRISPR/Cas9	(Gonzalez-Salinas et al., 2020) [54]
PTEN	SUM159	CRISPR activation	(Choudhury et al., 2016) [55]
CDK7	TNBC	CRISPR/Cas9 genetic editing	(Y. Wang et al., 2015) [56]
MASTL	Human Mammary tumor cell lines	CRISPR based interruption	(Álvarez-Fernández et al., 2018) [57]
MYC oncogene	_	CRISPR/Cas9 mediated mutagenesis	(Schuijers et al., 2018) [58]
CXCR7, CXCR4	MDA-MB231	CRISPR/Cas9 knockout	(S. Luo et al., 2016) [59]

The DNA endonucleases guided by RNA were developed by introducing the sgRNAs that were HPV specific into the px330 vector that was expressing S. pyogenes Cas9 to produce the px330-E6E7 plasmid, also known as E6E7, and were proven to be a successful approach. In HPV-positive cervical carcinoma cells, disruption of E6E7 led to apoptosis induction and tumor growth suppression [46]. Furthermore, it was revealed that combined treatment reduced the dosage of docetaxel used, and the CRISPR system’s attack decreased the adverse impact on other organs. DOC impact absorption into cells occurs significantly more quickly than CRISPR/ Cas9. Therefore, it would be advantageous to combine CRISPR therapy with conventional chemotherapy, so that the challenge of drug resistance and tumor inhibition be efficiently treated (Figure 3).

Figure 3. CRISPR cas9 in Reversing Drug Resistance in Cervical Cancer.

Kinesin-5 A133P mutation in ispinesib resistance in cervical cancer

A molecular motor protein called kinesin-5 is crucial for mitosis. An emerging body of research has revealed that kinesin-5 is involved in the development of tumors and is seen as a possible target for cervical cancer therapy [15]. Some of the molecules involved in resistance to cancer and their effects are briefly shown in Table 3.

Table 3. Cancer Therapeutic Agents' Resistance-mediating Molecules, as Discovered Using the CRISPR-Cas9 System.

Therapeutic agents	Molecules involved in resistance	Cancer	Results
Cytotoxic agents
Doxorubicin	P-glycoprotein	Breast cancer	Increases sensitivity to doxorubicin.
Epirubicin	MLL	Bladder cancer	Reverse drug resistance.
Cisplatin	p53, CTR	Oesophageal adenocarcinoma	Inhibits cell growth and induces cell cycle arrest.
Paclitaxel	Rsf-1	Lung cancer	Reverse drug resistance.
Immunotherapy
Cancer vaccines and adaptive T cell therapies	PBAF	Melanoma	Increases sensitivity to immunotherapy.
Molecular targeted agents
Ispinesib	Kinesin-5 A133P	Cervical cancer	Overthrows the previous resistance mechanism.
Imatinib	ASXL1	Chronicmyelocytic leukemia	Enhances differentiation ability.
Bortezomib	Rpn13	Multiple myeloma	Inhibits proliferation.
Trastuzumab	HER2	Breast cancer	Reverse drug resistance.
SAHA	p57	Pancreatic ductal adenocarcinoma	Reverse drug resistance.

Ispinesib, a kinesin-5 inhibitor, has started to be tested in human clinical trials as a cancer treatment. The over expression of kinesin-12 or activation of the EGFR, which were previously thought to be resistance mechanisms, did not contribute to Ispinesib resistance, according to a recent CRISPR-Cas9-based screening investigation.

CRISPR cas9 applications in cervical carcinogenesis

The abnormal proliferation of cells on the lining of cervix is what causes cervical cancer. In the type of cancers in women worldwide this type is the fourth most frequently occurring cancer. The most pervasive high-risk HPV type and the one that carries the highest risk of developing cervical cancer is human papillomavirus type 16 (HPV16). In their lifetime, more than 80% of women who have had at least one partner of the other sex will contract HPV [47]. It has very long been thought to be a major factor in the development of cervical cancer is because of high-risk HPV infection. The screening methods for cervical cancer shown by pap smears and the testing of high-risk HPV has shown some advances in recent years [48]. In most cases, surgical interventions were considered the prominent treatment for cervical cancer patients, but these had side effects on the patients and could also have a promising effect on the pregnancy of women for an extended period of time.

CRISPR cas9 system has proved to have favorable effects on cervical pre-cancer treatment and to the already present treatments of infections of cervix related to HPV, considered a promising restorative strategy [49]. The human papillomavirus (HPV) infection, which results in the integration of the virus’ oncogenes E6 and E7 into host cells, is the leading cause of cervical cancer. Therefore, the key to causing and maintaining cervical cell cancerization is the over expression of these two genes [50]. Many types of research have confirmed the role of HPV E6 and E7 oncogenes targeted by CRISPR cas system. While the E7 protein was genetically conserved, the HPV16 genome shared several amino acid-changing variations. Additionally, the E7 HPV16 is thought as the only oncoprotein that has the potential to immortalize human keratinocytes in vitro and induce cervical cancer in an animal model. It follows that HPV16 E7 that is targeted by CRISPR cas system may had interesting therapeutic applications (Figure 4).

Figure 4. Applications of CRISPR cas9 in Treatment of Cervical Carcinogenesis.

By this HPV16 E7 is considered as a suitable target for the HPV16-induced cervical cancer therapy. Additionally, in vitro and in naked mice models, gene therapy has shown encouraging effects, and theE6 gene of HPV16 is a strong aspirant cleavage site for this treatment.

Some of the gene editing tools like ZFN and TALENS have also shown the same efficacy as the CRISPR system in the treatment of cervical cancer [51]. The TALEN and ZFN are secluded proteins that have the flexible DNA restricting domain intertwined with FokI, that is an intricate design procedure for these tools to target a unique DNA sequence. The CRISPR system, in contrast, is reasonably straightforward method created to address this deficiency [52]. Additionally, because shRNA primarily target the products of transcription of the HPV16 oncogene, it had little effect on the HPV DNA that had been incorporated in the cells of human, indicating that such strategy could not be long lasting. Additionally, the CRISPR/Cas9 system may edit many genes simultaneously by creating different sgRNAs that attack various genomic sequences, which may significantly increase the technology’s therapeutic potential [53]. Therefore, it is believed that however, in some cell lines CRISPR along with some other gene editing tools has the same efficacy; it still is the more promising tool for the therapy of cervical lesions.

Future perspectives

The CRISPR cas9 system is thought to be the most useful for clinical protocols. Since it is discovered, CRISPR system has made it possible to investigate the function of specific genes in intricate biological processes. Technologies for CRISPR/Cas9-based screening have advanced, raising hopes for novel findings. Clinical trials for CRISPR/Cas9-based treatments, however, have been hampered by several issues, such as the possibility of off-target effects, the need to optimize attack sites, safety concerns while working in vivo, and a lack of available drug delivery options. For instance, techniques must be improved to transport the components of CRISPR/Cas9 safely and effectively in the targeted tissues. This genome editing technique has the potential to be dependable as well as simple to use. CRISPR/Cas9 has shown many ways for unveiling gene function in cancer treatment thanks to its simplicity and versatility. For example, current findings from genome-wide deep sequencing will be useful for choosing appropriate target sites and creating greatly specified gRNA. Additionally, interactions between CRISPR/Cas9 and additional chemo/radio therapeutic drugs may open new therapy options and enhance the clinical outcomes of cervical and breast cancer patients.

In conclusion, since scientists discovered they could use CRISPR to alter the DNA of any animal easily and precisely, it has revolutionized biology. It is a potent instrument that could be utilized to change the in a way that future generations could inherit the genome. Since its discovery, CRISPR/Cas9 has made it possible to investigate the function of specific genes in intricate biological processes. Technologies for CRISPR/Cas9-based screening have advanced, raising hopes for novel findings. For application in therapeutic studies; the CRISPR/Cas9 system enables precise editing of a target sequence in model organisms and humans. Theoretically, it is also conceivable to treat malignancies as well as genetic and viral illnesses. For cancer patients, the expansion of full genome libraries is made possible by CRISPR/Cas9, a very adaptable and practical method. CRISPR cas9 has proved to be the novel therapeutic strategy for the treatment of breast cancer and cervical cancer. Clinical results for patients with cervical and breast cancer may be improved by the interactions and alliances between CRISPR/Cas9 and other chemo/radio therapeutic medicines.

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