Copper Nanoparticles: A Review on Synthesis, Characterization and Applications
An emerging field of science “Nanotechnology” which is involved in manipulation of atoms and molecules has shown great potential in all fields of sciences. Nanotechnology deals with nanoparticles ranging from size 1 to 100 nm in diameter, due to small size and high surface area eventually increases the state of activity. This review focuses on metal and metal oxide nanoparticles and mainly on green synthesis, characterization and application of copper nanoparticles. Green synthesis of copper and copper oxide (Cu and CuO) is economically beneficial and ecofriendly. Copper nanoparticles are used in diverse fields such as biomedicine, pharmaceuticals, bioremediation, molecular biology, bioengineering, genetic engineering, dye degradation, catalysis, cosmetics and textiles. Structural properties and biological effects of copper nanoparticles have promising effectivity in field of life sciences.
Nanotechnology is an emerging field of science which involves change in matter at atomic or molecular level. Production of matters at a nanoscale is developed using Nanotechnology .This is a field of science which involves manipulation of atoms and molecules. A nanoparticle (NPs) is an ultrafine particle, a matter that ranges between 1 to 100 nm in diameter and with length of 1 to 1000 nm in one dimension . The size of nanoparticles depends on the method of reduction and its surrounding environment.
Nanoparticles are applicable in varied fields of biomedical and pharmaceutical like diagnostics, biomarkers, bio-imaging, cosmetics, antibacterial, anticancer, immunology, cardiology, genetic engineering, drug delivery for treating cancer and other infectious diseases, bioremediation (environmental applications), water treatments and energy production , , . Metal nanoparticles such as gold, copper, etc. have shown great approach in improving living standards and their need to be synthesized biologically has increased tremendously. Various metal and metal oxide as titanium dioxide (TiO2) and silicon dioxide are mostly used NPs in paints, to provide antifungal, anti-algal and antibacterial property to paints. AZO NANO also tell about Nano silver NPs showing antimicrobial property by binding to bacterial cell proteins, with deodorizing, hydrophobicity property and less toxic effect . These NPs have immense role in cosmeceuticals which gives therapeutically effect on skin, hairs also for treatment of photo aging, wrinkles, dark spots, etc. Various Nano carriers which encapsulate the drug to be targeted is formulated in cosmetics to show its effects . More specifically copper NPs are also used in making buildings, as reduces the roughness of steel which will give resistance to corrosion, great thermal conductivity, and heat transfer property, polymer-coated CuNPs shows antibacterial property, and also gives hydrophobicity property  .Recently there is development in synthesis of inorganic NPs includes all metal and metal oxide NPs (such as silver, gold, zinc oxide, copper oxide etc) which have shown wide range of application in all fields. Synthesis of NPs basically happens by two main methods Top down and Bottom up, in which green synthesis method of Bottom up approach is widely taken into consideration as synthesis method is ecofriendly, cost effective, and nontoxic way of developing nanoparticles . These NPs have wide range of application with all the field of sciences. So, this review will focus more on metal nanoparticles, with its green synthesis, characterization methods and applications.
There are various classifications of nanoparticles depending upon characteristics of material, dimensions, and origin , ,,.
1. Classification based on characteristics of material:
i. Carbon nanomaterials- It includes carbon nanotubes (CNTs), carbon nanofibers, fullerenes (C60) etc.
ii. Inorganic nanomaterials- It includes metal and magnetic nanoparticles like silver, gold, also metal oxides/ semi-channel NPs such as TiO2, ZnO, and CuO etc.
iii. Organic nanomaterials- It includes nanomaterials made from organic matter having weak interactions e.g. liposomes, dendrimers, micelles etc.
iv. Composite nanomaterials- These NPs are multiphase NPs which complicated structure of metal-organic frameworks. It may be combination of carbon, metal or organic with other metal or polymer.
2. Classification based on dimensions:
i. Zero-dimension nanoparticles- It includes quantum dots or quantum boxes.
ii. One-dimension nanoparticles- Polyethylene oxide nanofibers, Ag nanorods etc.
iii. Two-dimension nanoparticles- Carbon nanotubes, graphene nanosheets etc.
iv. Three-dimension nanoparticles- Dendrimers, fullerenes (C60), ZnO nanowires etc.
3. Classification based on origin:
i. Natural nanomaterials- NPs produced naturally by
biological species or naturally occurring on earth spheres.
ii. Synthetic nanomaterials- NPs which produced by reduction using various physical, chemical, biological or hybrid methodologies.
2. Metal & Metal Oxide Nanoparticles
There is increase in interest of metal nanoparticles due to their physical and chemical properties and wide area of application. There is increase in demand of metal, metal oxides, polymer nanoparticles etc. due to their small size and high surface area for interaction which has several uses in material science. Properties of nanoparticles depend on the surrounding medium and required properties could be introduced by altering the environment .Green syntheses of ZnO NPs shows varying morphologies and have more antimicrobial activity then chemically synthesized NPs . It has shown efficient inhibitory activity against E. coli,S. aureus, P. aeruginosa, S. thalpophilum, B. subtilis,
K. Pneumoniae and efficient hydroxyl radical scavenging antioxidant activity . Bovine serum albumin coated iron oxide nanoparticles carriers of curcumin were used for cytotoxic assay on HFF2 and MCF-7 breast cancer cell line showing positive results . Green synthesis of gold nanoparticles have proven to show antidiabetic activity by showing antioxidant activity which lead to anti-apoptotic property with decrease in level of Bcl-2 protein and increased level of Bax indicating cell survival induced by NF-κB in RIN-5F diabetic cell line . With significant inhibition in denaturation of BSA protein silver NPs shows anti-inflammatory activity .
There are several infectious microorganisms responsible for formation of Microbial biofilms that are sticky exopolymeric substances (EPS) causing adherence of microorganism to biotic surfaces such as host cells or abiotic surfaces such as medical devices cause antimicrobial resistance .Metal oxide nanoparticles are effective in fighting against multidrug resistance biofilm producing pathogenic bacteria, where CuO nanoparticles are found more effective than iron oxide and nickel nanoparticles ,. Antibacterial activity by copper and copper oxide NPs against biofilm producing organism Bacillus subtilis, Staphylococcus aureus, E. coli and Pseudomonas aeruginosa is shown ,. Effectivity of copper nanoparticles is found in varied applications such as Antifungal, Antiviral, Antibiotics, Anticancer, Photocatalytic, in biomedical, agriculture fields etc. and is cost effective .
3. Synthesis of Copper Nanoparticles
3.1. Physical and Chemical synthesis
Preparation of metal nanoparticles should be done using appropriate method to obtain a particular size of nanoparticle, as with use of particular method it reduces the size of particle and stabilizes it. Copper nanomaterials are greatly in attention due its profuse amount, availability and low cost in comparison to gold and silver, so large scale productions of copper nanoparticles are using various physical and chemical method . The major methods used for large scale production of nanoparticles is done through physical methods (Mechanical milling, laser ablation and sputtering) and chemical methods (Solid state, liquid state, gas phase, biological methods and other methods), terming them as Top down and Bottom up methods respectively . Top-down method for synthesis of nanoparticles is a method where bulk material is the initial material which is catabolized to reduce particle size, even though this methods are easy to perform but not suitable for preparing uniform size of particles and due to this it can affect the surface chemistry of NP’s ,. The mechanochemical method for synthesis of nanoparticles requires precursor for copper, salts for dilution and as starting material which are ball milled at ambient temperature, resulting into copper oxide (Ⅱ) nanoparticles enclosed into salt matrix further which were washed by distilled water in ultrasonic bath. These particles showed antimicrobial activity against S. aureus and E. coli . In one of the work iron metal as reducing agent with precursors, chalcocite (Cu2S) and covellite (CuS) were milled at different timings resulting into approx. 16 nm sizes of copper NPs proving to be a scalable method . Technique such as laser ablation targeting bulk copper with high power pulsed laser, with 20 mJ energy, 532 nm wavelength, 4 ns pulse width and power density of 5.24 × 1012 W cm-2 showing synthesis of copper oxide nanoparticles enhancing the growth of rice seedlings by hydroponics . Bottom-up approach for synthesis of nanoparticle is obtained through assembling of atoms, molecules or small particles giving Nano size dimension . Sol-gel method of using polymers are of great interest for green synthesis of nanoparticles, using Lantana camara extract, it was added to mixture of copper chloride and sodium hydroxide giving average particle size of 17 nm showing photocatalytic activity . Chemical reduction of copper ions with sodium borohydride and stabilization by polyvinylpyrrolidone (PVP) using copper chloride as precursor giving 7 nm size of NP’s by TEM and SEM analysis . Copper nanoparticles of range 38 to 50 nm were prepared using solvothermal reduction method with glycerol (reducing agent) and various surfactants for stabilizing the particles . The synthesis of NPs by thermal decomposition in liquid phase has achieved attention due to production of stable nanoparticle with easy method and also has shown antibacterial activity against E. coli ,. Microwave assisted green synthesis of CuO NPs was done using fruit extract of Myristica fragrans at 800 W and 2450 MHz frequency for 5 min with temperature below 100 °C with color change from blue to green was observed. The size of CuO NPs with TEM, SEM and XRD was found to be 4 nm, 13 nm and 15.7 nm respectively and showing antimicrobial and catalytic applications . Flame spray pyrolysis, a scalable method is flame assisted pyrolysis converting aerosol to vapor to producing fine and pure copper oxide (CuO) nanoparticles whose size can be managed with flame temperature, residence time and liquid precursor concentration for various applications , .
3.2. Green synthesis
An alternative method for synthesis of nanoparticles is “Green Synthesis” which is simple, cost effective, and reproducible, and gives stable product. This method does not require high energy, pressure, temperature, or toxic chemicals . Bottoms up approach for green synthesis is similar to that of chemical reduction of NPs, the difference is chemical reducing agent are replaced with extracts of plants, fruits, flower, and algae ,.
3.2.1. Plant and Fruit Extract mediated Synthesis
This process of synthesis begins by mixing the natural extracts with a metal solution; with biochemical reduction of salt color change is observed in the solution indicating synthesis of nanoparticles . Biosynthesis of copper nanoparticle using aqueous extract of Tilia and was added to copper sulfate pentahydrate solution with 4:1 (V/V), with continuous heating at 80 °C for 25 min; further precipitation and drying by putting into oven for 2 h at 100 °C was done. Hemispherical shaped with different diameter in range of 4.7-17.4 nm was observed by TEM studies. These NPs show antimicrobial and anticancer activities against human hepatic cancer (HepG2 cells) and breast cancer (Mcf-7 cells) .
Peppermint extract mediated synthesis of Cu NPs was coated with rifampicin (0.2 mg/mL) with constant stirring at 700 rpm for 5 hr maintaining; 6.5 pH & temperature between 25 and 45 °C, this enhanced the antimicrobial activity against Staphylococcus aureus, monitored using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM) assessing DNA cleavage by agarose gel electrophoresis . Copper oxide nanoparticle synthesis using Annona muricata leaf extract into copper (Ⅱ) sulphate with continuous stiring at 80°C for 12 hr followed by drying at 24 hr; further XRD, SEM and BIO-TEM analysis showed 30-40 nm size of CuO NPs, which shows photocatalytic and cytotoxic property . Fruit extract of Syzygium alternifolium was titrated with copper sulphate precursor (5 mM) at 50 °C for 2 hr, further precipitating at 10,000 RPM resulting into 17.5 nm average copper oxide particle size exhibiting antiviral activity against Newcastle Disease Virus (NDV) .
3.2.2. Bacterial and Fungal Mediated Synthesis
Microbial mediated synthesis of nanoparticles an evolving field of nanobiotechnology, involves certain mechanisms by which microorganisms thrive to grow with toxic metals which may lead to synthesis of nanoparticles as byproduct of reduction mechanism. As these microbes tend to produce enzymes which reduce the toxic metal resulting into formation of nanoparticles . CuO NPs biosynthesis using actinomycetes, a cell free supernatant was collected and added to 25 ml of CuSO4. 5H2O (10 mM) with heating at 100 °C for 15 min color change was observed. Further characteristics using XRD and TEM found 61.7 nm average size of NP which has potential antimicrobial property . Fungal mediated, cell free extract of Trichoderma asperellum was used to synthesize 10-190 nm range of copper oxide nanoparticles where in amide and aromatic groups of secondary metabolite was found to be as encapsulating or reducing agent determined by IR spectroscopy. This nanoparticle was also studied in vitro photothermal induced therapy of human lung carcinoma- A549 cancer cells . Agaricus bisporus (fungus) mediated green synthesis of copper NPs of size range 2- 10 nm which showed antimicrobial, antioxidant and cytotoxic activity against SW 620 colon cancer cells (Table 1) .
|Precursor||Reducing/ Oxidizing Agent||Size||Application||Reference|
|Copper sulphate||Ginger Lily leaf extract||40 nm Approx.||Antibacterial activity|||
|Copper Acetate Monohydrate||Psidium guajava leaf Extract||11.07 nm||Photocatalytic Dye Degradation|||
|Cupric Sulphate||Centella asiatica leaf extract||20-30 nm||Dye Degradation|||
|Cupric acetate||Camellia Sinensis leaf extract||22.44 nm Approx.||Antibacterial, cytotoxicity activity|||
|Copper Sulphate||Punica granatum leaf extract||20.33 nm||Dye Degradation|||
|Copper Sulphate||Sida acuta leaf extract||50 nm||Antibacterial, Dye degradation, Textile|||
|Copper Chloride||Tinospora cordifolia leaf extract||50-130 nm||Catalytic textile dye degradation|||
|Copper Acetate||Cissus quadrangularis leaf extract||30.08 nm||Antifungal activity|||
|Cupric Nitrate||Gloriosa superba L. leaf extract||5-10 nm||Antibacterial activity|||
|Copper nitrate trihydrate||Tinospora cordifolia leaf extract||6-8 nm||Photocatalytic, Antioxidant, Antibacterial activity|||
|Copper nitrate||Aloe vera leaf extract||22 nm||Antibacterial activity against fish pathogens|||
|Copper Sulphate||Tabernaemontana divaricate leaf extract||48 nm Approx.||Antibacterial activity against urinary tract pathogen|||
|Copper Sulphate||Strawberry Fruit extract (Stabilizing Agent- L ascorbic acid)||10-30 nm||Antioxidant, Antifungal, Antibacterial, Anticancer, cutaneous wound healing activity|||
|Copper sulphate||Terminalia bellirica fruit extract||2-7 nm||Antimicrobial activity|||
|Copper Sulphate||Citrus medica Linn. Fruit extract||10-60 nm||Antimicrobial activity|||
|Copper (Ⅱ) nitrate trihydrate||Cordia sebestena flower extract||20-35 nm||Photo degradation of Dyes, Antibacterial activity|||
|Copper Sulphate||Streptomyces spp. (microbes)||78 nm and 80 nm||Antimicrobial, Antifungal, Antioxidant activity, Larvicidal activity|||
|Copper (Ⅱ) chloride||Shewanella loihica PV-4 (microbes)||10-16 nm||Antibacterial activity|||
|Copper sulphate||Eichhornia crassipes leaf extract||28 nm Approx.||Antifungal activity against plant fungal pathogen|||
|Copper (Ⅱ) sulphate||Bifurcaria bifurcate brown alga extract||18.34 nm||Antimicrobial activity|||
3.2.3. Algal Mediated Synthesis
Phyconanotechnology, a study of algal mediated extracellular formation of nanoparticles is economical, ecofriendly, energy efficient and less-toxic method to reduce down the metal nanoparticles . Anabaena cylindrical (microalgae) extract was used for biosynthesis of CuO NPs with constant stirring at 60 °C with 500-1000 rpm rotational speed, resulted into particle size of 3.6 nm and this had a potential use in disinfection of drinking water .
The range of wavelength at which it reveals the synthesis of copper nanoparticle is of 200-800 nm by Surface Plasmon Analysis (SPR). UV-visible spectroscopy, SEM and TEM is used for determining shape, size and bandwidth, the crystal lattice structure is determined by XRD and presence of functional group on surface of nanoparticles can be determined by FTIR analysis . These are the majorly used characterization techniques for analysis of nanoparticles. Characterization of nanoparticles reveals about the different shapes, size and biological activity of nanoparticles changes with its structure. The particle size of nanoparticle plays an important role in many applications such as drug delivery and smaller the particle size larger the surface which could be targeted for drug release .
4.1 UV- Visible Spectrophotometry
This spectrophotometric technique is widely used for quantification of various transparent fluids. According to different absorption feature of an analyte UV-vis spectrometer analyses the concentration of the absorbing components. Due to various interaction of analyte with the surrounding solution with respect to time it can change the absorption spectra  this is observed in case of NPs. UV-vis spec. works on basic principle of Beer-Lambert Law of absorption and transmission of light to determine concentration of NPs. This technique is sensitive to change in concentration, size, and refractive index of NPs, as its varies due to change in pH and time of interaction with the solvent .
Optic and colloidal property of copper NPs was characterized by UV 3000+ LABINDIA double beam spectrophotometer with spectral range of 200 to 800 nm. Formation of CuNPs using Azadirachta indica leaves was observed with gradual color change of solution with different time, which was recorded by UV-vis spec. resulting in increase of SPR peak showing continuous reduction of copper ions to CuNPs . Bacillus cereus- mediated CuNPs synthesis was confirmed by SPR with size recorded between 570 and 620 nm further analysis showed presence of spherical shaped NPs .
3.3. XRD (X ray diffraction)
This method is used to determine crystal lattice structure, which resolves molecule at atomic level using constructive and destructive interference caused by the atom in lattice. The diffracted pattern of constructive interference by the crystal structure would be would be determined by d (spacing between planes of atoms) and reflection angle, θ which gives Bragg’s equation:
As XRD gives average of all crystal volume, peak broadening is seen which can be due to fine crystal structure, so the crystalline size D, can be found using Debye-Scherrer equation .
D8 Advance Bruker X-ray diffractometer with Cu Kα was used to study crystal lattice of CuNPs synthesized from Garcinia mangosteen leaf extract which resulted into 26.51 nm average particle size of NP which was calculated using Debye-Scherrer formula .
3.4. SEM Analysis
This method of characterization of NPs does surface analysis, which will enable us to know morphology, shape, size, chemical composition and orientation of materials. During SEM characterization the solution from of NPs is converted to dry powder form which is mounted on a sample holder, and fine beam of electron is passed on sample resulting in release of secondary electrons/ back scattered electrons which will determine surface of the sample . The release of electron from the nanomaterial varies according to its surface, due to which depression and elevation of surface can be analyzed enabling us to know the morphology of NPs.
The SEM analysis of CuNPs using different surfactants and precursor with varying reaction time was done using ZEISS EVO Series, Model EVO 18 microscope showing different shapes and size of crystal mainly focused, which concluded which increase in concentration of the reducing agent, the rate of reduction reaction increases .
3.5. TEM Analysis
This technique is used for analysis of physical properties such as size, morphology, and shape of NPs along with chemical compositions; it provides spatial resolution range of 1 to 100 nm resulting into 2D images. The resolution of microscopy depends on the accelerating voltage of primary electrons of range 100-300 kV. This method analyses 2D of a particle by estimating perpendicular electron beam but cannot estimate parallel beam, but 3rd dimension of particle could be analyzed using energy-filtered TEM and transmission electron tomography. The sample preparation of nano-material for analysis should be done precisely, for physicochemical analysis of NPs. With bright field imaging of TEM, the transmitted electrons from the specimen are analyzed whereas dark-filed imagining is due to diffracted electron. The image is recorded by CCD camera and fluorescent screen is used to view image .
The dry form of copper oxide NPs synthesized by Asamoah et.al., was re-suspended in proportionate water. This was further sonicated to avoid agglomeration of particles, and immobilizing the suspension on carbon grid and was analyzed after drying. Analysis resulted into formation of nanorods with average length and width of 100 nm and 14 nm respectively  showing antibacterial activity. TEM images taken by JEOL JEM- 1200EX microscope at 120kV using energy dispersive spectrometer (EDS) showed spherical shaped CuNPs and average size of NPs was found to be 15-20 nm detection using quasi elastic light scattering data (QELS) .
3.6. FTIR Analysis
This spectroscopy method enables us to know functional groups present on surface of NPs by measuring vibrational frequency of the bonding present in the functional group. The molecules responds to range of 1013-1014 Hz infrared radiation , which allows us to analyze the functional group such as carboxylate group, amino groups, phenolic group etc. So the extract used for reduction of CuNPs, the chemical components of those extracts which interacts with Cu ions this will result in attachment of functional groups on surface of NPs. This could be detected by FTIR analysis. Copper oxide synthesis using White-Rot Fungus (Stereum hirsutum) showing plasmon resonance between 590 to 650 nm with highest peak at 620 nm was observed, FTIR analysis done by CARY 630 FTIR Agilent Technologies resulted in band regions of 3280 cm-1 and 2924 cm-1 show presence of primary and secondary amines . The average size of copper NPs formed using Calotropis procera L. latex extract was found to be 20 nm using XRD and FTIR results showed role of free amino groups or carboxylate group in reduction and stabilization of CuNPs. This NPs possessed cytotoxicity activity against tumorous cells  . Seedless dates extract for synthesis of copper and copper oxide NPs revealed 8 peaks by FTIR analysis which represented presence of phenolic compound which may be acting as capping agent providing more stability 
Nanoparticles have potent applications in biomedical and pharmaceutical fields due to nano-size and high surface area. Major Fields of sciences which are benefited with CuNPs are covered below:
A) Biological Applications
Copper NPs shows antimicrobial activity towards Bacillus subtilis, E. coli, S. aureus, Micrococcus luteus, Pseudomonas aeruginosa, Salmonella enterica, and Enterobactor aerogenes ,, and also antifungal activity against Fusarium oxysporum and Phytophthora capsici . The copper ions are capable of damaging cell membrane, DNA, RNA and other molecules, thus Cu NPs have shown profound effect against viruses such as human influenza A (H1N1), avian influenza (H9N2), and many more including COVID-19 virus reducing its viability, and half-life .
Nanoparticles tend to elicit intrinsic and extrinsic apoptotic pathway for death of cancerous cells, and copper has found promising against cancer . Copper and copper oxide nanoparticles exhibit anticancerous activity against HeLa cells, MD A-MB-231 (human breast cancer cell lines), Caco-2 (human colon cancer cells), and HepG2 cells (Hepatic cancer cells) and Mcf-7 breast cancer cells ,, ,.
Wound healing property of CuNPs with significant increase in concentration of fibrocytes eventually forming collagen for repairing and wound contraction was seen with in vivo study on mouse . Cutaneous wound healing was also studied in vivo by synthesis of copper NPs by Falcaria vulgaris leaf extract and also showed potent cytotoxicity, antioxidant, antifungal, antibacterial activities .
Copper nanoparticles have improved antioxidant enzymes with decline of pro-inflammatory markers in CFA (complete Freund’s adjuvant- which mimics human arthritis outcome) stimulated arthritis in rats proving anti-inflammatory and anti-arthritic potentials .
UK manufacturer Promethane Particles along with textile companies and research institutes are developing Personal Protective Equipment (PPE) and are designing fabrics having Nano-copper into polymer fibers such as nylon, by melt extrusion process and developing antimicrobial fabrics which is under examination . Copper nanoparticles are incorporated in cotton fibers and antimicrobial evaluation is done against E. coli, S. aureus, Proteus vulgaris and K. pneumoniae , , which can be used in textiles to produce PPE.
C) Biocatalyst and Bioremediation
Copper being low cost metal, less toxicity and copper based catalyst can be recycled and reused again . Bioremediation of pollutant such as dyes has become important to treat the polluted water which can lead to alterations in aquatic life and can lead to dangerous outcome. So the properties of CuNPs are discussed for remediating polluted water bodies due to textile effluents, industrial disposal etc. Copper Oxide NPs for waste water purification  and the catalytic property of CuNPs was observed in reduction activity of Xanthene dye along with strong reducing agents which act as precursor of Cu2+ ions in formation of CuNPs which causes fluorescence quenching. These could also be applicable in biological sensing and bio-labelling . Degradation activity of CuO NPs was higer than Ni@Fe3O4 against organic dyes such as congo red, methylene blue and Rhodamine B  and also showed reduction of 4-nitrophenol  to treat Textile Wastewater. Aspergillus species responsible to produce aflatoxins (AFs) which is found to be carcinogenic, mutagenic etc., the adsorbent capacity of Cu-NPs for aflatoxin B1 was found to be more than Ag- NPs but less than Fe-NPs .
According to US NIH as: ‘Nanomedicine refers to highly specified medical intervention at the molecular scale for curing disease or repairing damaged tissues, such as bone, muscle, or nerve’ . Nanoparticles provide site specific drug delivering system, due to its small size, large absorptive surface for drug to carry and also shows improved therapeutic efficacy with less toxicity. Brest Cancer was targeted using chitosan coated copper nanoparticles at different pH for drug doxorubicin, on MCF-7 cell line by studying apoptotic activity of cells . Antimicrobial activity using biopolymer chitosan micro particles conjugated with copper NPs which shows greater synergic effect for antibacterial activity of tetracycline against Gram positive and Gram Negative bacteria  also core-shell Nano carriers were used to enhance activity of tetracycline, where copper oxide NPs was used as core and hyper-branched polyglycerol as shell .
E) Other Applications
Copper NPs have also shown profound applications in Food Packaging  and agriculture for crop improvement . Thus Copper nanoparticle shows wide range of application in field of biological, physical and chemical sciences.
In Conclusion, nanoparticle production from natural extracts is emerging widely in field of nanotechnology as greener the process better the outcome without any toxic effects. The main reason to use natural extracts is it becomes eco-friendly and free of chemical contaminants which has applications in major fields of sciences. A proper method, time, precursor, pH, temperature, incubation time must be taken into consideration to optimize the synthesis process. These methods are scalable, and more safe and stable then produced through physical and chemical procedures. Major synthesis of metal nanoparticles is observed due to their effective application.
Efforts are made to obtain secondary metabolites from the natural extracts at different concentrations, which act as reducing and stabilizing agent useful for capping. Also polymeric based nanoparticles are also emerging with development of nanoscience. This green synthesis methods are effective to reduce down the size of nanoparticle and extract itself stabilizes the NPs, thus this method is completely eco-friendly having considerable effects in showing antimicrobial, antifungal, anti-cancer, catalytic, textile, cosmetics, water remediation etc. and many more applications which opens door for many therapeutics and treatments. Due to the nature of biological entities with varied concentrations along with other organic agents will influence the size, shape and effectivity of NPs.
Various researches are being carried out to scale up green synthesis methods. This can fulfill the future aspects of demand for welfare of human.
- “Nanoparticles: Properties, applications and toxicities,” Khan I, Saeed K, Khan I. Arab. J. Chem.2019;12(7):908-931.
- Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations Jeevanandam Jaison, Barhoum Ahmed, Chan Yen S, Dufresne Alain, Danquah Michael K. Beilstein Journal of Nanotechnology.2018;9. CrossRef
- Extremophiles: Applications in nanotechnology: Biotechnological applications of extremophiles in nanotechnology Tiquia-Arashiro S, Rodrigues, D.F. 2016.
- Natural polymer drug delivery systems: Nanoparticles, plants, and algae Bhatia S. 2016.
- Medical Applications of Nanoparticles in Biological Imaging, Cell Labeling, Antimicrobial Agents, and Anticancer Nanodrugs Singh Ravina, Nalwa Hari Singh. Journal of Biomedical Nanotechnology.2011;7(4). CrossRef
- “Nanotechnology in the Paint Industry,” 2017. [Online] Cuffari B. Available: https://www.azonano.com/article.aspx?ArticleID=4710. [Accessed: 25-Jun-2020]..
- Role of Nanotechnology in Cosmeceuticals: A Review of Recent Advances Kaul Shreya, Gulati Neha, Verma Deepali, Mukherjee Siddhartha, Nagaich Upendra. Journal of Pharmaceutics.2018;2018. CrossRef
- “Morphology, large scale synthesis and building applications of copper nanomaterials,” Constr. Build. Mater Shah K.W, Lu Y. 2018;180:544-578.
- A biological synthesis of copper nanoparticles and its potential applications Santhoshkumar J, Agarwal H, Menon S, Rajeshkumar S, Venkat Kumar S. Elsevier Inc.2019.
- “A Review on Synthesis, Characterization and Applications of Copper Nanoparticles Using Green Method,” Rafique M, et al . Nano.2017;12(4).
- Synthesis, Characterization, and Applications of Copper Nanoparticles Din Muhammad Imran, Rehan Rida. Analytical Letters.2016;50(1). CrossRef
- Green synthesized ZnO nanoparticles against bacterial and fungal pathogens Gunalan Sangeetha, Sivaraj Rajeshwari, Rajendran Venckatesh. Progress in Natural Science: Materials International.2012;22(6). CrossRef
- “Green route for the synthesis of zinc oxide nanoparticles from Melia azedarach leaf extract and evaluation of their antioxidant and antibacterial activities,” Dhandapani K. V, et al . Biocatal. Agric. Biotechnol.2020;24:101517.
- “Bovine Serum Albumin (BSA) coated iron oxide magnetic nanoparticles as biocompatible carriers for curcumin-anticancer drug,” Nosrati H, Sefidi N, Sharafi A, Danafar H, Kheiri Manjili H. Bioorg. Chem.2018;76:501-509.
- Green synthesis of gold nanoparticle using Eclipta alba and its antidiabetic activities through regulation of Bcl-2 expression in pancreatic cell line Vijayakumar Shalini, Vinayagam Ramachandran, Anand Mariadoss Arokia Vijaya, Venkatachalam Karthikkumar, Saravanakumar Kandasamy, Wang Myeong-Hyeon, Casimeer C Sangeetha, KM Gothandam, David Ernest. Journal of Drug Delivery Science and Technology.2020;58. CrossRef
- “Green synthesis and characterization of silver nanoparticles using belladonna mother tincture and its efficacy as a potential antibacterial and anti-inflammatory agent,” Das P, Ghosal K, Jana N. K, Mukherjee A, Basak P. Mater. Chem. Phys.2019;228:310-317.
- “We are IntechOpen , the world ’ s leading publisher of Open Access books Built by scientists , for scientists TOP 1 %,” in Antimicrobials, Antibiotic Resistance, Antibiofilm Strategies and Activity Methods, Sahra Kırmusaoğlu, Ed. 2019 Sahra Kırmusaoğlu. .
- Comparative Study of Antibiofilm Activity of Copper Oxide and Iron Oxide Nanoparticles Against Multidrug Resistant Biofilm Forming Uropathogens Agarwala Munin, Choudhury Bula, Yadav R. N. S.. Indian Journal of Microbiology.2014;54(3). CrossRef
- Synthesis and biological function of Nickel and Copper nanoparticles Chaudhary Jyoti, Tailor Giriraj, Yadav B.L., Michael Oshon. Heliyon.2019;5(6). CrossRef
- Sugarcane juice mediated synthesis of copper oxide nanoparticles, characterization and their antibacterial activity Angeline Mary A.P., Thaminum Ansari A., Subramanian R.. Journal of King Saud University - Science.2019;31(4). CrossRef
- Green synthesis and characterizations of copper nanoparticles Ismail M.I.M.. Materials Chemistry and Physics.2020;240. CrossRef
- “Metal nanoparticles synthesis: An overview on methods of preparation, advantages and disadvantages, and applications,” Jamkhande P. G, Ghule N. W, Bamer A. H, Kalaskar M. G. J. Drug Deliv. Sci. Technol.2019;53:101174.
- Biogenic silver, gold and copper nanoparticles - A sustainable green chemistry approach for cancer therapy Vaid Prachi, Raizada Pankaj, Saini Adesh K., Saini Reena V.. Sustainable Chemistry and Pharmacy.2020;16. CrossRef
- Antibacterial activity of ultra-small copper oxide (II) nanoparticles synthesized by mechanochemical processing against S. aureus and E. coli Moniri Javadhesari S., Alipour S., Mohammadnejad S., Akbarpour M.R.. Materials Science and Engineering: C.2019;105. CrossRef
- Synthesis of copper nanoparticles from refractory sulfides using a semi-industrial mechanochemical approach Baláž Matej, Tešinský Matej, Marquardt Julien, Škrobian Milan, Daneu Nina, Rajňák Michal, Baláž Peter. Advanced Powder Technology.2020;31(2). CrossRef
- “Liquid assisted pulsed laser ablation synthesized copper oxide nanoparticles (CuO-NPs) and their differential impact on rice seedlings,” Tiwari P. K, et al . Ecotoxicol. Environ. Saf.2019;176:321-329.
- Biological synthesis of metallic nanoparticles Thakkar Kaushik N., Mhatre Snehit S., Parikh Rasesh Y.. Nanomedicine: Nanotechnology, Biology and Medicine.2010;6(2). CrossRef
- A sol-gel approach to the synthesis of CuO nanoparticles using Lantana camara leaf extract and their photo catalytic activity Arunkumar B., Johnson Jeyakumar S., Jothibas M.. Optik.2019;183. CrossRef
- “Synthesis of Cu nanoparticles by chemical reduction method,” AGUILAR M. S, ESPARZA R, ROSAS G. Trans. Nonferrous Met. Soc. China (English Ed.2019;29(7):1510-1515.
- “Affordable, Green, and Facile Synthesis of Copper Nanoparticles Stabilized by Environmentally Friendly Surfactants,” Metall. Mater. Trans Dobrovolný K, Ulbrich P, Švecová M, Bartůněk V. B Process Metall. Mater. Process. Sci..2015;46(6):2529-2533.
- Metal Nanoparticles: Thermal Decomposition, Biomedicinal Applications to Cancer Treatment, and Future Perspectives Odularu Ayodele Temidayo. Bioinorganic Chemistry and Applications.2018;2018. CrossRef
- Copper nanoparticles synthesized by thermal decomposition in liquid phase: the influence of capping ligands on the synthesis and bactericidal activity Effenberger Fernando B., Sulca Marcos A., Machini M. Teresa, Couto Ricardo A., Kiyohara Pedro K., Machado Giovanna, Rossi Liane M.. Journal of Nanoparticle Research.2014;16(11). CrossRef
- “Synthesis of silver and copper oxide nanoparticles using Myristica fragrans fruit extract: Antimicrobial and catalytic applications,” Sustain Sasidharan D, Namitha T. R, Johnson S. P, Jose V, Mathew P. Chem. Pharm.2020;16:100255.
- Copper oxide nanoparticle made by flame spray pyrolysis for photoelectrochemical water splitting – Part II. Photoelectrochemical study Chiang Chia-Ying, Aroh Kosi, Franson Nicholas, Satsangi Vibha Rani, Dass Sahab, Ehrman Sheryl. International Journal of Hydrogen Energy.2011;36(24). CrossRef
- Copper oxide nanoparticle made by flame spray pyrolysis for photoelectrochemical water splitting – Part I. CuO nanoparticle preparation Chiang Chia-Ying, Aroh Kosi, Ehrman Sheryl H.. International Journal of Hydrogen Energy.2012;37(6). CrossRef
- Green Synthesis of Nanomaterials Devatha C. P, Thalla A. K. Elsevier Ltd.2018.
- Green synthesis of nanoparticles and its potential application Hussain Imtiyaz, Singh N. B., Singh Ajey, Singh Himani, Singh S. C.. Biotechnology Letters.2015;38(4). CrossRef
- Green synthesis of nanoparticles: A greener approach for a cleaner future Pal G, Rai P, Pandey A. Elsevier Inc.2019.
- Biogenic copper nanoparticles and their applications: A review Al-Hakkani Mostafa F.. SN Applied Sciences.2020;2(3). CrossRef
- Biosynthesis of copper nanoparticles using aqueous Tilia extract: antimicrobial and anticancer activities Hassanien Reda, Husein Dalal Z., Al-Hakkani Mostafa F.. Heliyon.2018;4(12). CrossRef
- Green synthesis of rifampicin-loaded copper nanoparticles with enhanced antimicrobial activity Woźniak-Budych Marta J., Przysiecka Łucja, Langer Krzysztof, Peplińska Barbara, Jarek Marcin, Wiesner Maciej, Nowaczyk Grzegorz, Jurga Stefan. Journal of Materials Science: Materials in Medicine.2017;28(3). CrossRef
- “Eco-friendly cost-effective approach for synthesis of copper oxide nanoparticles for enhanced photocatalytic performance,” Kayalvizhi S, Sengottaiyan A, Selvankumar T, Senthilkumar B, Sudhakar C, Selvam K. Optik (Stuttg).2020;202:163507.
- Cost Effective, Green Synthesis of Copper Oxide Nanoparticles Using Fruit Extract of Syzygium alternifolium (Wt.) Walp., Characterization and Evaluation of Antiviral Activity Yugandhar Pulicherla, Vasavi Thirumalanadhuni, Jayavardhana Rao Yagani, Uma Maheswari Devi Palempalli, Narasimha Golla, Savithramma Nataru. Journal of Cluster Science.2018;29(4). CrossRef
- Microbial synthesis of nanoparticles and their potential applications in biomedicine Fariq Anila, Khan Tabeer, Yasmin Azra. Journal of Applied Biomedicine.2017;15(4). CrossRef
- “Biosynthesis, characterization and antibacterial activity of copper oxide nanoparticles (CuO NPs) from actinomycetes,” Nabila M. I., Kannabiran K. Biocatal. Agric. Biotechnol.2018;15:56-62.
- Biosynthesis and characterization of copper oxide nanoparticles from indigenous fungi and its effect of photothermolysis on human lung carcinoma Saravanakumar Kandasamy, Shanmugam Sabarathinam, Varukattu Nipun Babu, MubarakAli Davoodbasha, Kathiresan Kandasamy, Wang Myeong-Hyeon. Journal of Photochemistry and Photobiology B: Biology.2019;190. CrossRef
- “Agaricus bisporus mediated biosynthesis of copper nanoparticles and its biological effects: An in-vitro study,” Sriramulu M, Shanmugam S, Ponnusamy V. K. Colloids Interface Sci. Commun.2020;35(December 2019):100254.
- Algae as crucial organisms in advancing nanotechnology: a systematic review Sharma Aishwarye, Sharma Shruti, Sharma Kuldeep, Chetri Siva P. K., Vashishtha Amit, Singh Pushpa, Kumar Ravindra, Rathi Brijesh, Agrawal Veena. Journal of Applied Phycology.2015;28(3). CrossRef
- Disinfection of drinking water via algae mediated green synthesized copper oxide nanoparticles and its toxicity evaluation Bhattacharya Priyankari, Swarnakar Snehasikta, Ghosh Sourja, Majumdar Swachchha, Banerjee Sathi. Journal of Environmental Chemical Engineering.2019;7(1). CrossRef
- “Green Synthesis of Luminescent Copper Oxide Nanoparticles Using Ginger Lily Leaves Extract,” Nagore P. B, Ghoti A. J, Salve A. P , Mane K. G. J. Inorg. Organomet. Polym. Mater.2020.
- “Biogenic synthesis of copper oxide nanoparticles using plant extract and its prodigious potential for photocatalytic degradation of dyes,” Singh J, Kumar V, Kim K. H, Rawat M. Environ. Res.2019;177(February):108569.
- Degradation of dyes using biologically synthesized silver and copper nanoparticles Raina Swati, Roy Arpita, Bharadvaja Navneeta. Environmental Nanotechnology, Monitoring & Management.2020;13. CrossRef
- “Camellia sinensis leaf extract mediated synthesis of copper oxide nanostructures for potential biomedical applications,” Emima Jeronsia J, Allwin Joseph L, Annie Vinosha P, Jerline Mary A, Jerome Das S. Mater. Today Proc.2019;8:214-222.
- “Green synthesis of copper oxide nanoparticles using Punica granatum leaf extract applied to the removal of methylene blue,” Vidovix T. B, Quesada H. B, Januário E. F. D, Bergamasco R, Vieira A. M. S. Mater. Lett.2019;257:126685.
- Biogenesis of copper oxide nanoparticles (CuONPs) using Sida acuta and their incorporation over cotton fabrics to prevent the pathogenicity of Gram negative and Gram positive bacteria Sathiyavimal Selvam, Vasantharaj Seerangaraj, Bharathi Devaraj, Saravanan Mythili, Manikandan Elayaperumal, Kumar Smita S., Pugazhendhi Arivalagan. Journal of Photochemistry and Photobiology B: Biology.2018;188. CrossRef
- Green Synthesis of Colloidal Copper Nanoparticles Capped with Tinospora cordifolia and Its Application in Catalytic Degradation in Textile Dye: An Ecologically Sound Approach Sharma Prashansa, Pant Suman, Poonia Preeti, Kumari Smita, Dave Vivek, Sharma Swapnil. Journal of Inorganic and Organometallic Polymers and Materials.2018;28(6). CrossRef
- Cissus quadrangularis mediated ecofriendly synthesis of copper oxide nanoparticles and its antifungal studies against Aspergillus niger, Aspergillus flavus Devipriya Duraipandi, Roopan Selvaraj Mohana. Materials Science and Engineering: C.2017;80. CrossRef
- Green synthesis of CuO nanoparticles usingGloriosa superbaL. extract and their antibacterial activity Naika H. Raja, Lingaraju K., Manjunath K., Kumar Danith, Nagaraju G., Suresh D., Nagabhushana H.. Journal of Taibah University for Science.2015;9(1). CrossRef
- Tinospora cordifolia mediated facile green synthesis of cupric oxide nanoparticles and their photocatalytic, antioxidant and antibacterial properties Udayabhanu , Nethravathi P.C., Pavan Kumar M.A., Suresh D., Lingaraju K., Rajanaika H., Nagabhushana H., Sharma S.C. Materials Science in Semiconductor Processing.2015;33. CrossRef
- “Green Synthesis of Copper Oxide Nanoparticles Using Aloe vera Leaf Extract and Its Antibacterial Activity Against Fish Bacterial Pathogens,” Kumar P. P. N. V, Shameem U, Kollu P, Kalyani R. L, Pammi S. V. N. Bionanoscience.2015;5(3):135-139.
- Biogenic copper oxide nanoparticles synthesis using Tabernaemontana divaricate leaf extract and its antibacterial activity against urinary tract pathogen Sivaraj Rajeshwari, Rahman Pattanathu K.S.M., Rajiv P., Salam Hasna Abdul, Venckatesh R.. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.2014;133. CrossRef
- Synthesis, characterization, and evaluation of cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing effects of copper nanoparticles using the aqueous extract of Strawberry fruit and l-Ascorbic acid Hemmati Saba, Ahmeda Ahmad, Salehabadi Yaser, Zangeneh Akram, Zangeneh Mohammad Mahdi. Polyhedron.2020;180. CrossRef
- Fine Ultrasmall Copper Nanoparticle (UCuNPs) Synthesis by Using Terminalia bellirica Fruit Extract and Its Antimicrobial Activity Viswadevarayalu Annavaram, Venkata Ramana Posa, Sreenivasa Kumar Godlaveeti, Rathna sylvia Lodi, Sumalatha Jorepalli, Adinarayana Reddy Somala. Journal of Cluster Science.2015;27(1). CrossRef
- Green synthesis of copper nanoparticles by Citrus medica Linn. (Idilimbu) juice and its antimicrobial activity Shende Sudhir, Ingle Avinash P., Gade Aniket, Rai Mahendra. World Journal of Microbiology and Biotechnology.2015;31(6). CrossRef
- Green synthesis of copper oxide nanoparticles and its effective applications in Biginelli reaction, BTB photodegradation and antibacterial activity Prakash Shanmugam, Elavarasan Nagaraj, Venkatesan Alagesan, Subashini Kasivisvanathan, Sowndharya Murugesan, Sujatha Venugopal. Advanced Powder Technology.2018;29(12). CrossRef
- Endophytic actinomycetes Streptomyces spp mediated biosynthesis of copper oxide nanoparticles as a promising tool for biotechnological applications Hassan Saad El-Din, Fouda Amr, Radwan Ahmed A., Salem Salem S., Barghoth Mohammed G., Awad Mohamed A., Abdo Abdullah M., El-Gamal Mamdouh S.. JBIC Journal of Biological Inorganic Chemistry.2019;24(3). CrossRef
- “Biosynthesis of copper nanoparticles using Shewanella loihica PV-4 with antibacterial activity: Novel approach and mechanisms investigation,” Lv Q, et al . J. Hazard. Mater.2018;347(2010):141-149.
- Synthesis and characterization of Eichhornia-mediated copper oxide nanoparticles and assessing their antifungal activity against plant pathogens VANATHI P, RAJIV P, SIVARAJ RAJESHWARI. Bulletin of Materials Science.2016;39(5). CrossRef
- Biosynthesis, characterization and antimicrobial activity of copper oxide nanoparticles (CONPs) produced using brown alga extract (Bifurcaria bifurcata) Abboud Y., Saffaj T., Chagraoui A., El Bouari A., Brouzi K., Tanane O., Ihssane B.. Applied Nanoscience.2013;4(5). CrossRef
- A. G. Shard, R. C. Schofield, and C. Minelli, Ultraviolet-visible spectrophotometry Elsevier Inc.2019;1.
- Green synthesis and characterization of copper nanoparticles using Azadirachta indica leaves Nagar Niharika, Devra Vijay. Materials Chemistry and Physics.2018;213. CrossRef
- Extracellular biosynthesis of copper sulfide nanoparticles by Shewanella oneidensis MR-1 as a photothermal agent Zhou Nan-Qing, Tian Li-Jiao, Wang Yu-Cai, Li Dao-Bo, Li Pan-Pan, Zhang Xing, Yu Han-Qing. Enzyme and Microbial Technology.2016;95. CrossRef
- “Textbook of Nanoscience and Nanotechnology,” Murty B. S, Shankar P, Raj B, Rath B. B, Murday J. Textb. Nanosci. Nanotechnol.2013;:149-175.
- A facile biosynthesis of copper nanoparticles: A micro-structural and antibacterial activity investigation Prabhu Y.T., Venkateswara Rao K., Sesha Sai V., Pavani Tambur. Journal of Saudi Chemical Society.2017;21(2). CrossRef
- “Synthesis and Characterization of Oleic Acid-Capped Metallic Copper Nanoparticle via Chemical Reduction Method,” Arif Z, Soni A. B, Victoria S. N, Manivannan R. J. Inst. Eng. Ser. E.2019;100(1):101-109.
- Characterization of nanomaterials by transmission electron microscopy: Measurement procedures Mast J, Verleysen E, Hodoroaba V. D, Kaegi R. Elsevier Inc.2019.
- Synthesis and characterization of zinc and copper oxide nanoparticles and their antibacteria activity Asamoah R.B., Yaya A., Mensah B., Nbalayim P., Apalangya V., Bensah Y.D., Damoah L.N.W., Agyei-Tuffour B., Dodoo-Arhin D., Annan E.. Results in Materials.2020;7. CrossRef
- Synthesis and characterization of copper nanoparticles by reducing agent Chandra Sulekh, Kumar Avdhesh, Tomar Praveen Kumar. Journal of Saudi Chemical Society.2014;18(2). CrossRef
- “Using Infrared Spectroscopy to Study Self Assembled Monolayers,” Ltd S. 2017.
- Extracellular Biosynthesis of Copper and Copper Oxide Nanoparticles byStereum hirsutum, a Native White-Rot Fungus from Chilean Forests Cuevas R., Durán N., Diez M. C., Tortella G. R., Rubilar O.. Journal of Nanomaterials.2015;2015. CrossRef
- Novel route for rapid biosynthesis of copper nanoparticles using aqueous extract of Calotropis procera L. latex and their cytotoxicity on tumor cells Harne Shrikant, Sharma Ashwinikumar, Dhaygude Mayur, Joglekar Shreeram, Kodam Kisan, Hudlikar Manish. Colloids and Surfaces B: Biointerfaces.2012;95. CrossRef
- Green synthesis of copper & copper oxide nanoparticles using the extract of seedless dates Mohamed Elwy A.. Heliyon.2020;6(1). CrossRef
- “Green synthesis of Cu nanoparticles using Curcuma longa extract and their application in antimicrobial activity,” Jayarambabu N, Akshaykranth A, Venkatappa Rao T, Venkateswara Rao K, Rakesh Kumar R. Mater. Lett.2020;259:126813.
- Biogenesis of copper nanoparticles using peel extract of Punica granatum and their antimicrobial activity against opportunistic pathogens Kaur Pawan, Thakur Rajesh, Chaudhury Ashok. Green Chemistry Letters and Reviews.2016;9(1). CrossRef
- “Materials Today : Proceedings Green synthesis of copper oxide nanoparticles using Cedrus deodara aqueous extract for antibacterial activity,” Ramzan M, Obodo R. M, Mukhtar S, Ilyas S. Z, Aziz F, Thovhogi N. Mater. Today Proc., no. xxxx.2020.
- “Preparation and characterization of antifungal colloidal copper nanoparticles and their antifungal activity against Fusarium oxysporum and Phytophthora capsici,” Pham N. D, Duong M. M, Le M. V, Hoang H. A., Pham L. K. O. Comptes Rendus Chim.2019;22(11-12):786-793.
- The role of additive manufacturing and antimicrobial polymers in the COVID-19 pandemic Zuniga Jorge M., Cortes Aaron. Expert Review of Medical Devices.2020;17(6). CrossRef
- “PROMETHEAN TESTING COPPER NANOPARTICLES AGAINST VIRUSES,” 2020. [Online] Available: https://www.technicaltextile.net/news/promethean-testing-copper-nanoparticles-against-viruses-266670.html. [Accessed: 20-Jun-2020]..
- “Synthesis of ecofriendly copper oxide nanoparticles for fabrication over textile fabrics: Characterization of antibacterial activity and dye degradation potential,” Vasantharaj S, et al . J. Photochem. Photobiol. B Biol.2019;191(November 2018):143-149.
- Green synthesis: In-vitro anticancer activity of copper oxide nanoparticles against human cervical carcinoma cells Nagajyothi P.C., Muthuraman P., Sreekanth T.V.M., Kim Doo Hwan, Shim Jaesool. Arabian Journal of Chemistry.2017;10(2). CrossRef
- Bioinspired green synthesis of copper oxide nanoparticles from Syzygium alternifolium (Wt.) Walp: characterization and evaluation of its synergistic antimicrobial and anticancer activity Yugandhar Pulicherla, Vasavi Thirumalanadhuni, Uma Maheswari Devi Palempalli, Savithramma Nataru. Applied Nanoscience.2017;7(7). CrossRef
- Green synthesis of copper oxide nanoparticles using sinapic acid: an underpinning step towards antiangiogenic therapy for breast cancer Raj Preeth Desingh, Shairam Manickaraj, Suganya Natarajan, Hootan Roshandel, Kartik Ravishankar, Pierre Kennepohl, Suvro Chatterjee, Rajalakshmi Subramaniyam. JBIC Journal of Biological Inorganic Chemistry.2019;24(5). CrossRef
- Green synthesis and chemical characterization of copper nanoparticles using Allium saralicum leaves and assessment of their cytotoxicity, antioxidant, antimicrobial, and cutaneous wound healing properties Tahvilian Reza, Zangeneh Mohammad Mahdi, Falahi Homeyra, Sadrjavadi Komail, Jalalvand Ali R., Zangeneh Akram. Applied Organometallic Chemistry.2019;33(12). CrossRef
- Novel synthesis of Falcaria vulgaris leaf extract conjugated copper nanoparticles with potent cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing activities under in vitro and in vivo condition Zangeneh Mohammad Mahdi, Ghaneialvar Hori, Akbaribazm Mohsen, Ghanimatdan Mohamad, Abbasi Naser, Goorani Samaneh, Pirabbasi Elham, Zangeneh Akram. Journal of Photochemistry and Photobiology B: Biology.2019;197. CrossRef
- Biogenic nanoparticles: copper, copper oxides, copper sulphides, complex copper nanostructures and their applications Rubilar Olga, Rai Mahendra, Tortella Gonzalo, Diez Maria Cristina, Seabra Amedea B., Durán Nelson. Biotechnology Letters.2013;35(9). CrossRef
- “l P of,” Opt. - Int. J. Light Electron Opt M. Rafique , et al . 2020;:p. 165138.
- “Catalytic and fluorescence studies with copper nanoparticles synthesized in polysorbates of varying hydrophobicity,” Mandal S, De S. Colloids Surfaces A Physicochem. Eng. Asp.2015;467(1):233-250.
- “Green synthesis of Ni@Fe3O4 and CuO nanoparticles using Euphorbia maculata extract as photocatalysts for the degradation of organic pollutants under UV-irradiation,” Pakzad K, Alinezhad H, Nasrollahzadeh M. Ceram. Int.2019;45(14):17173-17182.
- Green synthesis of copper oxide nanoparticles/clinoptilolite using Rheum palmatum L. root extract: high catalytic activity for reduction of 4-nitro phenol, rhodamine B, and methylene blue Bordbar Maryam, Sharifi-Zarchi Zeinab, Khodadadi Bahar. Journal of Sol-Gel Science and Technology.2016;81(3). CrossRef
- “Iron, copper and silver nanoparticles: Green synthesis using green and black tea leaves extracts and evaluation of antibacterial, antifungal and aflatoxin B1 adsorption activity,” Asghar M. A, et al., . LWT - Food Sci. Technol.2018;90(June 2017):98-107.
- Nanostructured pH-responsive biocompatible chitosan coated copper oxide nanoparticles: A polymeric smart intracellular delivery system for doxorubicin in breast cancer cells Varukattu Nipun Babu, Vivek Raju, Rejeeth Chandrababu, Thangam Ramar, Ponraj Thondhi, Sharma Alok, Kannan Soundarapandian. Arabian Journal of Chemistry.2020;13(1). CrossRef
- Novel synergistic activities of tetracycline copper oxide nanoparticles integrated into chitosan micro particles for delivery against multiple drug resistant strains: Generation of reactive oxygen species (ROS) and cell death Assadi Zahra, Emtiazi Giti, Zarrabi Ali. Journal of Drug Delivery Science and Technology.2018;44. CrossRef
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- “Jo ur l P re of,” Saravanakumar K, Sathiyaseelan A, Vijaya A, Mariadoss A, Xiaowen H, Wang M. Int. J. Biol. Macromol.2020.
- “Jo ur l P re of,” Wang Y, et al . Sci. Total Environ.2020;:p. 138387.
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