Improved Antitumor Efficacy of Liposome-Encapsulated  Selenium Nanoparticles

Mahya Asadalizadeh; Hamid Ghahremani; Parizad Ghanbarikondori; Helia Asadalizadeh; Parham Rahmani; Fatemeh Rostamian Motlagh

doi:10.31557/apjcb.2025.10.2.323-331

Authors

Mahya Asadalizadeh Department of Biomedical Engineering, College of Engineering, University of Illinois at Chicago, Chicago, IL, USA.
Hamid Ghahremani Radio_ oncology Department Science Valiasr Hospital Zanjan University Medical Science, Zanjan, Iran.
Parizad Ghanbarikondori Department of Pharmaceutics, Pharmaceutical Sciences Branch, Islamic Azad University (IAU), Tehran, Iran.
Helia Asadalizadeh Department of Biology, Harold Washington College, City College of Chicago, Chicago, IL, USA.
Parham Rahmani Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Fatemeh Rostamian Motlagh Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran.

DOI:

https://doi.org/10.31557/apjcb.2025.10.2.323-331

Keywords:

Anticancer activity, Oral cancer therapy, Liposomes

Abstract

Overview: This investigation chronicles the phytogenic synthesis of selenium nanoparticles (SeNPs) and their subsequent entrapment within phospholipid liposomes to construct a precision nanovector for oral-squamouscell-carcinoma therapy.

Methods: The plant-derived SeNPs were loaded into liposomes via a thin-film hydration approach. Dynamic light scattering (DLS) assessed their hydrodynamic diameter and zeta potential.

Results: This nanoparticle was then sequestered via a thin-film-hydration protocol that yielded liposomes with a mean hydrodynamic diameter of 235 nm, a polydispersity index of 0.15. Dynamic-release profiling in phosphate-buffered saline (pH 7.4, 37 °C) revealed a sustained discharge of 35 % of the payload over 62 h dramatically slower than the 95 % burst exhibited by free SeNPs attesting to the kinetic moderation conferred by the bilayer matrix. Functionally, MTT assays on an oral-cancer cell line demonstrated a 72 % reduction in viability after 24 h, significantly eclipsing the 38 % inhibition achieved by unencapsulated nanoparticles (p < 0.001).

Conclusion: These data indicate that liposomal sequestration furnishes SeNPs with enhanced colloidal stability, protracted release dynamics, and markedly elevated in-vitro antineoplastic potency, thereby positioning the platform as a compelling, biocompatible candidate for targeted oral-cancer therapeutics and warranting subsequent in-vivo validation.