Association of NQO1 C609T (Pro187Ser) with Risk of Oral Submucous Fibrosis in Eastern Indian Population

Authors

  • Sanjit Mukherjee CSIR-Indian Institute of Chemical Biology, Kolkata, India
  • Sweta Mohanty Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Atul Katarkar CSIR-Indian Institute of Chemical Biology, Kolkata, India. University of Lausanne, Epalinges-1066, Switzerland
  • Richa Dhariwal Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Basudev Mahato Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Jay Gopal Ray Department of Oral Pathology, Dr. R. Ahmed Dental College & Hospital, Kolkata, India
  • Keya Chaudhuri CSIR-Indian Institute of Chemical Biology, Kolkata, India.

DOI:

https://doi.org/10.31557/apjcb.2021.6.3.187-193

Keywords:

Areca nut, Oral submucous fibrosis, NQO1, single nucleotide polymorphism, carcinogenesis.

Abstract

Objective: Oral submucous fibrosis (OSF) is a debilitating disease mainly attributed to chewing areca nut with a 7.4-13% malignant transformation rate. The present study explores the role of NADPH quinone oxidoreductase 1 (NQO1) C609T (Pro187Ser) polymorphism in susceptibility to OSF among habitual areca nut chewers in an eastern Indian population.
Material and Methods: In this hospital-based study, 152 controls and 179 OSF cases were genotyped at NQO1 C609T polymorphic site by PCR-RFLP and its effect on NQO1 expression in OSF tissue was studied to determine the risk of the disease.
Results: Overall, about 18% of the total OSF cases were detected carrying minor TT alleles (Ser/Ser) p=0.026. When categorized by age, both CT (Pro/Ser) and TT (Ser/Ser) alleles were significantly higher (p= 0.003 & 0.004 respectively) in cases above 40years of age. NQO1 protein was 42% reduced in buccal tissues of heterozygous (Pro/Ser) carriers, whereas a 70% reduction was observed in TT (Ser/Ser) OSF cases.
Conclusion: Our study suggests that the NQO1 C609T polymorphism confers increased risk for OSF in habitual chewers. 

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Published

2021-07-09

How to Cite

1.
Mukherjee S, Mohanty S, Katarkar A, Dhariwal R, Mahato B, Ray JG, et al. Association of NQO1 C609T (Pro187Ser) with Risk of Oral Submucous Fibrosis in Eastern Indian Population. Asian Pac J Cancer Biol [Internet]. 2021 Jul. 9 [cited 2026 Jun. 14];6(3):187-93. Available from: http://waocp.com/journal/index.php/apjcb/article/view/683

Issue

Vol. 6 No. 3 (2021)

Section

Research Articles/ Original Work

License

 West Asia Organization for Cabcer Prevention retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License 4 (This permits anyone to copy, distribute, transmit and adapt the published work, provided the original work and source are appropriately cited).